After submission, a validation page shows detailed information about tested gene(s) and:
number of patients from original studies tested,
1 complete data before filtering;
2 if the gene is found or not;
and 3 patients finally analysed (if no missing genomic data).
A summary is given at the bottom of the page, you can then choose to validate (by hitting "Start analysis" button) or cancel your submission.
"Cancel" will redirect you back to previous screen, and offer you to choose new criteria on the form.
Results are presented with all box plots showing distribution of gene expression according to the different groups.
For each criterion, click on the "box plot" button, on the right, to display or hide the figure.
In this tutorial, NPI results were chosen.
For age criterion, the case 2 [≤51 / >51 years old] was determined by the age at menopause in western industrialised countries.
Global significant difference between groups is assessed by Welch's test, the corresponding p-value is indicated on the bottom right of the figure.
If a global significant difference exists (p < 0.05) and there are more than 2 groups, a Dunnett-Tukey-Kramer's test is computed for each pairwise comparison.
The Dunnett-Tukey-Kramer's test table is presented below the figure.
The colour of the p-value depends on the result: the p-value is written in green if a significant difference exists (p < 0.05),
in orange if there is a trend (p = 0.05-0.10)
and in red if there is no difference (p > 0.10).